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plates  (R&D Systems)


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    Structured Review

    R&D Systems plates
    Plates, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/streptavidin+coated+plates/EvenCoat+Streptavidin+Coated+Plates/us12600698-861-3-4
    Average 94 stars, based on 19 article reviews
    plates - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Human gut bacteria produce structurally related monoglycolipids with contrasting immune functions
    Article Snippet: .. After overnight incubation at 37°C, ligand-loaded CD1d (0.25 ug per well) was bound onto streptavidin-coated plates (R&D Systems #CP003). ..

    Article Title: Human gut bacteria produce structurally related monoglycolipids with contrasting immune functions.
    Article Snippet: Gut symbiont Bacteroides fragilis can produce α-galactosylceramides (BfaGCs), sphingolipids with immunomodulatory functions that regulate colonic natural killer T (NKT) cells.. However, their synthesis pathway and whether other human gut bacteria can produce them are unclear.. Here, using genetic and metabolomic approaches, we mapped the sphingolipid biosynthesis pathway of B. fragilis and determined that α-galactosyltransferase (agcT) is essential and sufficient for colonic NKT cell regulation in mice.

    Recombinant:

    Article Title: Hydrophobic interactions dominate the recognition of a KRAS G12V neoantigen
    Article Snippet: Purified scDbs were analyzed using a TSKgel G3000SWxl column (TOSOH Bioscience) with 50 mM sodium phosphate 300 mM sodium chloride running buffer, pH 7, and a flow rate of 1 ml/min. scDbs were quantified by spectrophotometry (Nanodrop, Thermo Fisher Scientific). .. The streptavidin-coated plates (R&D Systems, Minneapolis, MN) were coated with 50 μl of 0.5 μg/ml of biotinylated KRAS G12V [7–16]/HLA-A3 monomer, 0.5 μg/ml biotinylated KRAS WT [7–16]/HLA-A3 monomer, or 0.4 μg/ml biotinylated recombinant human CD3ε/CD3δ heterodimer (CDD-H82W6, Acro Biosystems, Newark, DE) in BAE buffer (PBS containing 0.5% bovine serum albumin [Sigma], 2 mM EDTA [Thermo] and 0.1% sodium azide) for 1 h at room temperature. .. Plates were washed 6 times with 1× TBST (J77500-K8, Thermo Fisher Scientific) using a 405 TS microplate washer (BioTek, Winooski, VT). scDb samples were diluted to 200 ng/ml in PBS and incubated in the coated plates for 1 h at room temperature.

    Article Title: Hydrophobic interactions dominate the recognition of a KRAS G12V neoantigen.
    Article Snippet: For SPR, scDbs were expressed in Expi293 cells (Thermo Fisher Scientific, A14527) by GeneArt (Thermo Fisher Scientific) and purified with a HisTrap column (GE Healthcare) followed by size exclusion chromatography using a HiLoad Superdex 200 16/600 column (GE Healthcare). .. Purified scDbs were analyzed using a TSKgel G3000SWxl column (TOSOH Bioscience) with 50mM sodium phosphate 300mMsodiumchloride running buffer, pH 7, and a flow rate of 1ml/min. scDbs were quantified by spectrophotometry (Nanodrop, Thermo Fisher Scientific). scDb monomer ELISAs The streptavidin-coated plates (R&D Systems, Minneapolis, MN) were coated with 50μl of 0.5μg/ml of biotinylated KRASG12V[7–16]/HLA-A3 monomer, 0.5μg/ml biotinylated KRASWT[7–16]/HLA-A3 monomer, or 0.4μg/ml biotinylated recombinant human CD3ε/CD3δ heterodimer (CDD-H82W6, Acro Biosystems, Newark, DE) in BAE buffer (PBS containing 0.5% bovine serum albumin [Sigma], 2mM EDTA [Thermo] and 0.1% sodium azide) for 1 h at room temperature. .. Plates were washed 6 times with 1× TBST (J77500-K8, Thermo Fisher Scientific) using a 405 TS microplate washer (BioTek, Winooski, VT). scDb samples were diluted to 200 ng/ml in PBS and incubated in the coated plates for 1 h at room temperature.

    Labeling:

    Article Title: A cytoplasmic motif in HLA-E that drives clathrin-mediated endocytosis and VCP-associated postendocytic trafficking.
    Article Snippet: .. N- terminal biotin- labeled peptides (SI Appendix, Table S1) were diluted to 5 μg/mL in DPBS, followed by 50 μL of this dilution was immobilized onto streptavidin- coated plates (RandD Systems, CP003) for 1 h at RT. .. After washing five times with DPBS (300 μL/each), plates were blocked with 300 μL blocking buffer (2% IgG- free bovine serum albumin (Gibco) in DPBS) for 2 h at RT.

    Purification:

    Article Title: Hydrophobic interactions dominate the recognition of a KRAS G12V neoantigen.
    Article Snippet: For SPR, scDbs were expressed in Expi293 cells (Thermo Fisher Scientific, A14527) by GeneArt (Thermo Fisher Scientific) and purified with a HisTrap column (GE Healthcare) followed by size exclusion chromatography using a HiLoad Superdex 200 16/600 column (GE Healthcare). .. Purified scDbs were analyzed using a TSKgel G3000SWxl column (TOSOH Bioscience) with 50mM sodium phosphate 300mMsodiumchloride running buffer, pH 7, and a flow rate of 1ml/min. scDbs were quantified by spectrophotometry (Nanodrop, Thermo Fisher Scientific). scDb monomer ELISAs The streptavidin-coated plates (R&D Systems, Minneapolis, MN) were coated with 50μl of 0.5μg/ml of biotinylated KRASG12V[7–16]/HLA-A3 monomer, 0.5μg/ml biotinylated KRASWT[7–16]/HLA-A3 monomer, or 0.4μg/ml biotinylated recombinant human CD3ε/CD3δ heterodimer (CDD-H82W6, Acro Biosystems, Newark, DE) in BAE buffer (PBS containing 0.5% bovine serum albumin [Sigma], 2mM EDTA [Thermo] and 0.1% sodium azide) for 1 h at room temperature. .. Plates were washed 6 times with 1× TBST (J77500-K8, Thermo Fisher Scientific) using a 405 TS microplate washer (BioTek, Winooski, VT). scDb samples were diluted to 200 ng/ml in PBS and incubated in the coated plates for 1 h at room temperature.

    Spectrophotometry:

    Article Title: Hydrophobic interactions dominate the recognition of a KRAS G12V neoantigen.
    Article Snippet: For SPR, scDbs were expressed in Expi293 cells (Thermo Fisher Scientific, A14527) by GeneArt (Thermo Fisher Scientific) and purified with a HisTrap column (GE Healthcare) followed by size exclusion chromatography using a HiLoad Superdex 200 16/600 column (GE Healthcare). .. Purified scDbs were analyzed using a TSKgel G3000SWxl column (TOSOH Bioscience) with 50mM sodium phosphate 300mMsodiumchloride running buffer, pH 7, and a flow rate of 1ml/min. scDbs were quantified by spectrophotometry (Nanodrop, Thermo Fisher Scientific). scDb monomer ELISAs The streptavidin-coated plates (R&D Systems, Minneapolis, MN) were coated with 50μl of 0.5μg/ml of biotinylated KRASG12V[7–16]/HLA-A3 monomer, 0.5μg/ml biotinylated KRASWT[7–16]/HLA-A3 monomer, or 0.4μg/ml biotinylated recombinant human CD3ε/CD3δ heterodimer (CDD-H82W6, Acro Biosystems, Newark, DE) in BAE buffer (PBS containing 0.5% bovine serum albumin [Sigma], 2mM EDTA [Thermo] and 0.1% sodium azide) for 1 h at room temperature. .. Plates were washed 6 times with 1× TBST (J77500-K8, Thermo Fisher Scientific) using a 405 TS microplate washer (BioTek, Winooski, VT). scDb samples were diluted to 200 ng/ml in PBS and incubated in the coated plates for 1 h at room temperature.



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